28-04-2026 20:07
Lothar Krieglsteiner
... on twig in the air at standing Ceratonia siliq
14-04-2026 05:32
Ethan CrensonHi all, A few weeks back a friend pointed out som
28-04-2026 20:33
Vitus SchäfftleinHello, I found Trochila ilicina on Ilex aquifoliu
30-04-2026 10:28
Rot BojanHello, by appearance I would say that I am dealing
27-04-2026 18:48
Tony MoverleyCollected 23rd April 2026, Norfolk, EnglandSwarms
27-04-2026 20:52
Lothar Krieglsteiner
Found on hanging tiwg of Olea europaea in dried-ou
28-04-2026 22:51
Bernard CLESSE
Bonsoir à toutes et tous,Pourriez-vous m'aider à
29-04-2026 08:01
Lothar Krieglsteiner
... on twig attached to small tree of Citrus auran
29-04-2026 10:44
Lothar Krieglsteiner
growing at moist, drying-out soil at the side of a
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
