30-04-2024 16:22
François BartholomeeusenDear forum members,On April 25 2024, I found one f
01-05-2024 23:22
Ethan CrensonHi all, Found late last week in a New York City p
29-04-2024 21:32
Robin IsakssonHi! Found in Sweden. Ascomata with haris, se
01-05-2024 12:54
F. JAVIER BALDA JAUREGUIHello, everyone.An idea for this pyreno, I found u
30-04-2024 19:43
Gernot FriebesHi!We observed this hyphomycete growing between le
29-04-2024 21:51
Mathias HassHi everyone, Found on attached branches of top pa
28-04-2024 18:05
Bernard CLESSEBonsoir à toutes et tous,J'ai trouvé ce matin ce
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.