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17-12-2017 10:54

Per Marstad Per Marstad

Hello.This was found on sticks of Fagus.Spores 11-

17-12-2017 13:17

Blasco Rafael Blasco Rafael

Hola, de nuevo necesito su ayuda con esta muestra,

17-12-2017 12:55

Blasco Rafael Blasco Rafael

Hola, les parece que sea la que propongo ????Recog

14-12-2017 11:51

Per Marstad Per Marstad

3-8 mm in diameter, black, sp. 14-15 x 7-8 my, asc

16-12-2017 09:57

Castillo Joseba Castillo Joseba

Me mandas esta muestra seca en caña de PhytolacaL

16-12-2017 14:18

Per Marstad Per Marstad

I would like a name on this. 2 mm in diameter, dar

16-12-2017 20:58

Mlcoch Patrik Mlcoch Patrik

Good day,I want ask you about help with determinat

15-12-2017 15:20

BERNARD CLESSE BERNARD CLESSE

Dans le même xérobrometum (pelouse calcicole xé

14-12-2017 11:51

Per Marstad Per Marstad

3-8 mm in diameter, black, sp. 14-15 x 7-8 my, asc

14-12-2017 14:19

Castillo Joseba Castillo Joseba

En Phytolaca americana, me mandan el material sec

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"Discomycetes" - 293285
Danny Newman, 05-10-2017 06:10
Danny Newmanasci inamyloid

Spores: 1-septate


(9.3) 9.6 – 12.7 (15) × (2.8) 3.2 – 4 (4.4) µm
Q = (2.5) 2.6 – 3.6 (4.7) ; N = 35
Me = 11.1 × 3.6 µm ; Qe = 3.1


12.19 3.37
9.86 3.75
10.87 4.08
13.47 3.33
9.76 3.19
9.54 3.84
9.57 3.58
9.58 3.32
11.01 3.56
10.52 3.48
14.48 3.06
11.49 3.64
9.72 3.10
9.47 3.38
10.68 3.34
11.03 3.34
14.67 4.10
10.79 3.86
12.39 3.99
12.25 3.66
10.68 3.63
10.09 3.46
9.92 3.49
12.35 4.05
12.66 3.76
15.04 4.39
9.69 3.44
9.72 3.80
10.49 3.58
10.62 4.04
10.62 3.60
9.99 3.35
12.07 2.83
9.32 3.70
10.89 3.16


Substrate: overmature polypore


Habitat: mixed woods (maple, birch, hemlock, pine, cedar)


Ecoregion: Eastern Forest Boreal-Transition (NA0406)


Collectors: D. Newman


Collected during the 2017 Peck Foray held at the SUNY ESF Newcomb Campus in Newcomb, New York.

also seen at:

https://www.facebook.com/groups/ascomycetes/permalink/1895848247334037/
http://mushroomobserver.org/293285


NOTE: I cannot shrink my images down to 150KB to be uploaded here. they may be viewed at either of the two above links.

Hans-Otto Baral, 05-10-2017 06:51
Hans-Otto Baral
Re : "Discomycetes" - 293285
Sorry but in MLZ I cannot identify fungi. 

Looks for me like Calycina (Bisporella) citrina which may occasionally go on polypores. 
But asci should show a reaction, even in MLZ (clearer the reaction would perhaps be in IKI).

Is the excipulum gelatinised and cartilaginous?
Jason Karakehian, 05-10-2017 17:33
Jason Karakehian
Re : "Discomycetes" - 293285
I have collected this a few times now - Maine, Massachusetts and New Brunswick. I determined it to B. citrina. These collections were on old, rotten Dadaleopsis confragosa polypores and on the surrounding wood. Korf believed that B. citrina was a parasite of this. See Korf and Carpenter, Mycotaxon 1(1) p. 57. 1974. I attach a few photos from different collections here.
  • message #50633
  • message #50633
Hans-Otto Baral, 05-10-2017 18:39
Hans-Otto Baral
Re : "Discomycetes" - 293285
This is a problematic case.

First: The type of Bisporella, B. pallescens (Helotiaceae), is not parasitic on Bispora, but Bispora is, unlike Fuckel and Korf & Carpenter assumed, the anamorph (we proved that in culture but did no publish the result except for the mere citation of this relationship in Syllabus 2016. 

Second: Bisporella is heterogeneous, a majority belonging to Calycina (Pezizellaceae), including citrina and sulfurina/claroflava. These two species are indeed facultatively fungicolous. For instance, the type of Parthenope parasitica Velen., which is a synonym of C. citrina, grew on a polypore.

Zotto

Danny Newman, 05-10-2017 18:45
Danny Newman
Re : "Discomycetes" - 293285
reading this:

http://www.gbif-mycology.de/HostedSites/Baral/IodineReaction.htm
https://invivoveritas.de/articles/iodine-reaction-in-ascomycetes-why-is-lugols-solution-superior-to-melzers-reagent/


and realizing the err of my ways. Is Baral's Solution preferable to Lugol's? How is it made/where can it be purchased?


Many thanks for your conitnued assistance.  Will look at the excipulum next.


-Danny

Hans-Otto Baral, 05-10-2017 19:01
Hans-Otto Baral
Re : "Discomycetes" - 293285
Thanks for looking at my homepage. Well, European reagent sellers have it, and it is applied here by many. Some even ascribe it to me (in the higher concentration), but it was a French physician who introduced it.

The antiseptic Lugol I was thinking is in alcohol, which is not suitable for the present purpose. Lugol has usually a weak concentration (0.3%), but when I look here
http://en.academic.ru/dic.nsf/enwiki/751372/Lugol%27s_iodine
I see a concentration over 5%!

The solution should be deep brown, minimum 0.5%, not looking yellow in a glass bottle. Either you buy crystalline iodine and KI and mixed it in tap water, or you buy the solution.
Zottto
Danny Newman, 05-10-2017 19:08
Danny Newman
Re : "Discomycetes" - 293285
Hans-Otto Baral, 05-10-2017 19:25
Hans-Otto Baral
Re : "Discomycetes" - 293285
It actually seems so! The answer on the question of the dosage on bottle was:

each drop contains 1 mg iodine and 1.5 mg potassium iodide,

which is actually exactly the formula for Lugol.

1g iodine in 1 l water would be 1%, the optimum concentration.

1000 drops of this Lugol are certainly not 1 l, so the concentration must be very high in this reagent. But you can easily dilute it.

Good is to keep a high concentrated solution behind because iodine escapes slowly from the solution via plastic lids.
Danny Newman, 05-10-2017 20:06
Danny Newman
Re : "Discomycetes" - 293285
i'm not sure I understand.  the formula is the exact same as the ideal Lugol's used in mycological applications, but it still needs to be diluted?  if so, how would it best be diluted?  one drop distilled water, one drop Lugol's?
Hans-Otto Baral, 05-10-2017 21:10
Hans-Otto Baral
Re : "Discomycetes" - 293285
The relation between I2 and KI is exactly the same as in the current Lugol (1 : 3), but the concentration seems to be so high that it probably should be diluted by adding maybe 5-10 parts of water to 1 part of the solution. Of course I would dilute a small quantity only.