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07-05-2024 00:04

Ethan Crenson

A friend found these black gelatnous cups on a twi

07-05-2024 19:26

Louis DENY

Bonsoir forum Sur tige de rubus fruticosus de l'a

06-05-2024 08:27

Blasco Rafael Blasco Rafael

Hola Buenos días.Alguno de ustedes tiene disponib

06-05-2024 10:02

François Bartholomeeusen

Good morning,At the end of an excursion in De Zegg

05-05-2024 12:55

Sylvie Le Goff

Bonjour Peut on dire que les poils soient vitreux?

05-05-2024 09:59

Gernot Friebes

Hello,I failed to identify this anamorph, which gr

19-04-2015 20:20

Enrique Rubio Enrique Rubio

Hi again Could you help me with this paper? NANN

30-04-2024 16:22

François Bartholomeeusen

Dear forum members,On April 25 2024, I found one f

03-05-2024 18:04

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde on Rubus fr

02-05-2024 20:04

éric ROMERO éric ROMERO

Bonjour, Une question à propos de la réaction a

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tiny black gelatinous ascos
Ethan Crenson, 07-05-2024 00:04
A friend found these black gelatnous cups on a twig (might have been Vitus, maybe not).  Unfortunatley they are not fully mature yet. When found they were dried and appeared to have collapsed in on themselves. When rehydrated they show a black receptical and a gelatinous yellow-brown hymenium.

The ectal excipulum seems to be textura porrecta—long undulating, slightly roughened, brown cells that run in parallel and are septate.  The ends sometimes have unusual looking tongue-like heads.

Spores are still developing in the asci, but appear to be broadly ellipsoid and might ultimately have one oil drop. I noticed at least one spore that was septate (last photo).

Paraphyses filiform.  

I am keeping these in a moist chamber, hoping they will mature. But in the meantime, does anyone have an idea what they might be?
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Hans-Otto Baral, 07-05-2024 09:09
Hans-Otto Baral
Re : tiny black gelatinous ascos
You mean Vitis probably. The fungus appears to be drought-tolerant, even the submature ascus looks as if alive. Did you test IKI? The apical thickening might ger much thicker when dead and perhaps stained.

The spores look multiguttulate with a central nucleus (did you mean this with the one oil drop?).

The excipulum reminds me of a Godronia. You could test KOH for a ionomidotic reaction which occurs in Godronia.
Ethan Crenson, 07-05-2024 20:57
Re : tiny black gelatinous ascos
Interesting.  Yes, Vitis was what I meant. Also, it seemed to me that the spores were not fully developed and the circle at the center would emerge as an oil droplet. I am not always certain how to discern if there is a visible nucleus. 

Fortunately, the specimen matured a bit and now I have more information. 

Spores:
1-septate, finely warted, broadly ellipsoid, some having a gelatinous sheath in the asci. In Lugol's the septum sometimes appears sinuate. In some asci the four lower spores seem to develop to full maturity before the upper four spores:
19.5-23.5 x 8.2-11.3µm
Q 1.9-2.5
N=15
Me = 20.8 x 9.6µm

Asci:
IKI-, clavate, spores uniseriate, in Cotton Blue the walls do thicken
232-393 x 16-22.7µm


Only faint yellow pigments in KOH after more than 5 minutes (no ionomidotic reaction).
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Hans-Otto Baral, 07-05-2024 22:53
Hans-Otto Baral
Re : tiny black gelatinous ascos
Yery good! At the moment I can only find Dermatea viticola in my database but spores are smaller and Dermatea has angularis.
Ethan Crenson, 07-05-2024 23:45
Re : tiny black gelatinous ascos
Dermatea doesn't seem to be a very common genus. It resolves to Dermea on iNaturalist.  I'm afraid I'm a bit lost.  Also, I may be completely wrong about the substrate being Vitis as I mentioned in my first note. I guess I should try to sequence this.