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Technical question regarding Bombardia
Martin Bemmann, 02-08-2010 23:49
Martin BemmannHi,

did someone of you try to make a slide of Bombardia? I find it very hard 1. to get a good section and 2. to get a thin preparation due to the "bouncy" texture of the perithecium. Would KOH be of any help?
I hope, this is a Bombardia... (picture attached) ;-)

All the best,

Martin
  • message #12456
Christian Lechat, 03-08-2010 05:32
Christian Lechat
Re:Technical question regarding Bombardia
Hi Martin,
I think your fungus is not Bombardia, it resembles to Coryne, asexual state of Ascocoryne.

If you want to make a section, it is necessary to dry the specimen before.
Regards,
Christian
Michel Delpont, 03-08-2010 09:53
Michel Delpont
Re:Technical question regarding Bombardia
Hi Martin.

I also think that Christian to reason, and that it is not Bombardia;
Regards.
Michel.
Martin Bemmann, 03-08-2010 11:16
Martin Bemmann
Re:Technical question regarding Bombardia
Dear Christian and Michel,

sorry to have shown you a macro only, that might have been not so typical for the habitus of Bombardia. I have seen this fungus never before. I attache some more macros and micros.

  • message #12461
Martin Bemmann, 03-08-2010 11:17
Martin Bemmann
Re:Technical question regarding Bombardia
another closer view:

  • message #12462
Martin Bemmann, 03-08-2010 11:18
Martin Bemmann
Re:Technical question regarding Bombardia
Asci with unmature spores:

  • message #12463
Martin Bemmann, 03-08-2010 11:20
Martin Bemmann
Re:Technical question regarding Bombardia
I did not find free spores, but my colleague did (who made this collection). Here is her picture of it.

Best regards,

Martin
  • message #12464
Jacques Fournier, 03-08-2010 15:43
Jacques Fournier
Re:Technical question regarding Bombardia
Hi Martin,
the shape of the ascomata are not typical, they even apparently lack a porate ostiole on the photo taken from above, but asci and spores might correspond to this species.
You should check two important things:
first the thick gelatinous layer in the wall . To help make sections try embedding a fresh or rehydrated ascoma in PVA -l actophenol, set it lying on the side and make sections with a new razor blade when it is dry (about 2 days at room temperature). PVA is available through Marcel Lecomte website, link somewhere on Ascofrance.
second, your collection seems immature, incubate it in a plastic box until you get mature ascospores with a brown swollen head.
A very good description and illustrations of this species are in Lundqvist 1972.
Good luck,
Jacques
Martin Bemmann, 03-08-2010 16:48
Martin Bemmann
Re:Technical question regarding Bombardia
Hi Jaques,

thank you for your oppinion. I attach a macro of the apical area of the ascomata.
Before I started this thread I tried to make a section of a perithecium embedded in polyethylenglycole (is PVA something else?). The bad result you can see in the next attachment. The perithecium was shrinking in the hot PEG. Maybe I should try to dissolve the PEG in water instead of heating it.

Best regards,

Martin
  • message #12467
Martin Bemmann, 03-08-2010 16:49
Martin Bemmann
Re:Technical question regarding Bombardia
section:
  • message #12468
Martin Bemmann, 06-08-2010 23:50
Martin Bemmann
Re:Technical question regarding Bombardia
Interim report:

Hi everybody,

beeing in captivity in a moist environment for some days now, some ascomata deloped a more distinct ostiolum. Some have discharched spores that are maturing now outside the asci but adhering in lumps. I harvested some of these lumps and show you some pictures below. I already saw two mature brown ovoid spore cells so typical for Bombardia.
I did not have the time to make a preparation for a proper section but I just cut one specimen in half. It shows a very thick gelatinous wall.
I can't find any recent literature to this genus, but I think I can file it as Bombardia bombarda/fasciculata. Obviously it is not found/collected so often (cf. Verbreitungsatlas der Großpilze Deutschlands 2) and therefore variance in habitus is not so well known.
I eagerly wait for well developed spores. If I find them, I will show to complete this thread.

Best regards,

Martin

pictures start with ostioles:
  • message #12482
Martin Bemmann, 06-08-2010 23:51
Martin Bemmann
Re:Technical question regarding Bombardia
next lumps of spores (sorry for bad quality of picture):
  • message #12483
Martin Bemmann, 06-08-2010 23:52
Martin Bemmann
Re:Technical question regarding Bombardia
then microscopy of some of them:
  • message #12484
Martin Bemmann, 06-08-2010 23:52
Martin Bemmann
Re:Technical question regarding Bombardia
more
  • message #12485
Martin Bemmann, 06-08-2010 23:53
Martin Bemmann
Re:Technical question regarding Bombardia
even more, sorry
  • message #12486
Martin Bemmann, 06-08-2010 23:53
Martin Bemmann
Re:Technical question regarding Bombardia
and last the above mentioned section:
  • message #12487
Peter Welt, 07-08-2010 14:49
Peter Welt
Re:Technical question regarding Bombardia
Glückwunsch zu diesen schönen Fund Martin. Typisch ist für Lasiosphaeriacaen die späte Entwicklung der Sporen. Die einzigen Funde der mir aus Deutschland bekannt sind, sind die von Batsch 1785 und der von Fuckel (Nass. Jahrbücher 23-24: 164) Jahr ? aber vor 1869.

Gruß Peter

Michel Delpont, 07-08-2010 17:41
Michel Delpont
Re:Technical question regarding Bombardia
Hi Martin

Congratulations again for this discovery. Bombardia is a rare kind, I have never found, and unfortunately did not have much documentation to help you find the specie. I hope someone can do.
Regards.
Michel.
Martin Bemmann, 07-08-2010 17:57
Martin Bemmann
Re:Technical question regarding Bombardia
Hi Peter and Michel,

actually I did not find it, as I mentioned above it was my workmate who found it and gave it to me for determination. So credits are to her and I will tell her...

Cheers,

Martin