Accès membres

Mot de passe perdu? S'inscrire

07-05-2024 00:04

Ethan Crenson

A friend found these black gelatnous cups on a twi

06-05-2024 10:02

François Bartholomeeusen

Good morning,At the end of an excursion in De Zegg

07-05-2024 19:26

Louis DENY

Bonsoir forum Sur tige de rubus fruticosus de l'a

06-05-2024 08:27

Blasco Rafael Blasco Rafael

Hola Buenos días.Alguno de ustedes tiene disponib

05-05-2024 12:55

Sylvie Le Goff

Bonjour Peut on dire que les poils soient vitreux?

05-05-2024 09:59

Gernot Friebes

Hello,I failed to identify this anamorph, which gr

19-04-2015 20:20

Enrique Rubio Enrique Rubio

Hi again Could you help me with this paper? NANN

30-04-2024 16:22

François Bartholomeeusen

Dear forum members,On April 25 2024, I found one f

03-05-2024 18:04

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde on Rubus fr

02-05-2024 20:04

éric ROMERO éric ROMERO

Bonjour, Une question à propos de la réaction a

« < 1 2 3 4 5 > »
Pyrenopeziza species
Nicolas VAN VOOREN, 01-07-2012 17:05
Nicolas VAN VOORENI cannot determine this collection made on Rumex dead stems.

Apothecia subglobose then cupulate, Ø 0.2-1 mm, grey brown to greyish, outer surface with brown hairs, and margin with white hairs.
Outer excipulum composed of textura subglobulosa with brown cells, Ø 6-12 µm. Lateral hairs pale brown, septate, 55-120 x 4.7-5.5 µm, partially covered by an amorphous process. Marginal hairs hyaline, 3-4septate, 52-65 x 4-5 µm.
Spores subfusoid, 10-13 (14) x (2) 2.3-3 µm, eguttulate, in some cases slightly curved.
Asci 40-50 x 6-8 µm, without crozier, 8-spored, pars spor. 20-27 µm, with apical ring IKI+ (bb)
Paraphyses hyaline, with few small vacuoles.

The closest species seems to be Pyrenopeziza escharodes but it has smaller spores and asci with crozier. Any idea?
  • message #19077
Hans-Otto Baral, 01-07-2012 17:18
Hans-Otto Baral
Re : Pyrenopeziza species
Reminds me a bit of P. dilutella. But that has guttulate spores and croziers. Are you sure with the absence of croziers? This is quite ?a rare feature in Pyrenopeziza.

Zotto
Andreas Gminder, 01-07-2012 17:22
Andreas Gminder
Re : Pyrenopeziza species
Hello Nicolas,

I have found the same species some days ago, I think. But I have not noticed the absence of crosiers, I have to check that.
My collections was very abundant on dead stems of Rumex, associated with Pyrenopeziza escharodes. In fact they were growing side by side, but could be separated by the hairy margin of the first one, whereas P. escharodes had a nearly smooth margin. I show fotos when I have reduced them to a reasonable size.
The spore size of my collection was even bigger: (11)12-16 x 2,5-3 µm, oil absent. Marginal hairs hyalin, 30-60 µm long, up to 4-celled.
I took samples for DNA-extraction. Location was Obergurgl in Austria, in 1900 m NN.

best regards,
Andreas
Andreas Gminder, 01-07-2012 17:36
Andreas Gminder
Re : Pyrenopeziza species
Hello,

here is a first foto of the collection, showing the Pyrenopeziza spec with fringent margin and apothecia colour from whitish to grey, as well as apothecia of Pyrenopeziza escharodes showing blackish apothecia with a white (+/- smooth) margin.

best regards,
Andraes
  • message #19080
Nicolas VAN VOOREN, 01-07-2012 18:17
Nicolas VAN VOOREN
Re : Pyrenopeziza species
Thanks Andreas. I think also this is the same species.
My collection was made in Switzerland, near Fribourg (Moléson Mountain), with Michel Hairaud.
There were also other ascomata, more greyish, but they were parasited by Helicogonium trabinelloides.
About the croziers, if they are present, I couldn't distinct them.
Michel Hairaud, 01-07-2012 21:06
Michel Hairaud
Re : Pyrenopeziza species
Bonsoir Nicolas, Zotto, Andreas et tous,

I did collect   this  species  on dead black petioles of Rumex ( alpinus I suppose)  and tried to study it with no name to put on it and I even got a doubt about the genera as the paraphyses appeared to be full after a while of Vbs staining in CRB (which is not a character, as far as I know, in Pyrenopeziza) 
My main data are :
Ascus 52-63 (67) x 6-7, H+, iki intense blue
Spores 10,5-14 x 2-2,8 (3) , G =0, cylindro clavate
EE globulosa angularis 7-15 (25) , dark walls
ME globulosa with smaller cells 5-10 µm
Margin hairs up to 85 x 4-6µm  and 5x septate, hyalin to dark , merging in ''locks''
Anchoring hyphae 1,5-2, dark braun

Here are a few pics macro and micro, I can send more if necessary 

Amitiés
Michel


  • message #19082
  • message #19082
  • message #19082
  • message #19082
  • message #19082
  • message #19082
Hans-Otto Baral, 01-07-2012 21:29
Hans-Otto Baral
Re : Pyrenopeziza species
Hi all

Clearly a Pyrenopeziza, Michel. F. ex. the ascus shape with a elongate-conical apex. Pyrenopeziza may have globose low-refractive VBs as far as I recall. The fourth pic is strange, however, but it is obviously an alteration induced by IKI, maybe distorted SCBs?

You don't have free spores? I see some small LBs near the poles. Any CRB-image?

Zotto
Andreas Gminder, 01-07-2012 21:38
Andreas Gminder
Re : Pyrenopeziza species
Hello,

I have seen in a few spores one very small oil drop directly at the pole.

best regards,
Andreas
Michel Hairaud, 01-07-2012 21:48
Michel Hairaud
Re : Pyrenopeziza species
Hi Zotto, 
Here are the pics you asked for .
Andreas and Nico, did you notice the same vacuoles in paraphyses?

Michel
  • message #19086
  • message #19086
Hans-Otto Baral, 01-07-2012 22:13
Hans-Otto Baral
Re : Pyrenopeziza species
Fine! Yes, I think sich globose VBs are possible in Pyrenopeziza.
Zotto
Raúl Tena Lahoz, 02-07-2012 11:23
Raúl Tena Lahoz
Re : Pyrenopeziza species
Hi all!

I can´t say anything about the Rumex Pyrenopeziza but here I attach a photo of what I believe is Pyrenopeziza urticicola. In this one I saw mixed VBs and SCBs inside paraphyses. VBs were nearly non-refractive, while SCBs were more refractive. VBs stained in CRB whereas SCBs remained unstained.
Michel, did you notice SCBs in your paraphyses? 

I wanted to ask:
Presence of SCB in paraphyses and hairs is a character of Pyrenopeziza? Is it a good character and can it be used to distinguish Pyrenopeziza from Mollisia?

Best regards, 
Raúl
  • message #19089
Hans-Otto Baral, 02-07-2012 11:58
Hans-Otto Baral
Re : Pyrenopeziza species
Oh yes, that's important: VBs can also be elongate in Pyrenopeziza. And yes, I never saw SCBs in Mollisia.

Hairs are not necessary in the genus, but frequent, sometimes very short.
Zotto
Andreas Gminder, 02-07-2012 12:02
Andreas Gminder
Re : Pyrenopeziza species
Hello,

Vb can indeed be somewhat elongate in Pyrenopeziza, but they are never that continuous then in Mollisia. I think one can say that their length-width-ratio is never more then 2 or may be 3.
I have never observed Pyrenopezizas in cresyl blue, to be honest. I will do from now on.

best regards,
Andreas
Raúl Tena Lahoz, 02-07-2012 12:21
Raúl Tena Lahoz
Re : Pyrenopeziza species
Thanks Zotto!
Merci Andreas!

Raúl
Michel Hairaud, 02-07-2012 13:10
Michel Hairaud
Re : Pyrenopeziza species
Bonjour cher Raul, 

Thanks for your contribution, I did not notice SCBs in the paraphyses of this collection but I must confess I do not search for these bodies in Pyrenopeziza, which I will try to do from now on .

Amitiés (et bravo pour l'actualité sportive !)

Michel

Raúl Tena Lahoz, 02-07-2012 13:51
Raúl Tena Lahoz
Re : Pyrenopeziza species
Merci pour les donnés Michel!
Pour l'actualité sportive: c´est le tiki-taka ;-)))

Amitiés,
Raúl