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21-03-2019 11:16

Castillo Joseba Castillo Joseba

En excrementos de caballoA ver que os pareceSaludo

21-03-2019 11:52

Joop van der Lee

Found on deer dung on two different sites.One coll

21-03-2019 11:32

Michel RIMBAUD

Bonjour,Récolté il y a quelques jours sur bois p

21-03-2019 09:09

Andrés Valverde Valera Andrés Valverde Valera

Bonjour, Je vous envoie des photos de cet ascomyc

18-03-2019 23:10

Patrice TANCHAUD

Bonsoir, récolte réalisée sous pins (seul coni

21-03-2019 01:15

Valencia Lopez Francisco Javier

Hola amigosAdjunto fotos macro/micro de unos Ascob

19-03-2019 17:12

Per Marstad Per Marstad

We have found this to day, on Betula pubescens.Ca.

18-03-2019 19:41

Per Marstad Per Marstad

We have found this to-day, in Norway, ca. 1 mm in

18-03-2019 21:48

Ethan Crenson

Hello again,On the same branch as my previous post

17-03-2019 22:26

Ethan Crenson

Found yesterday in New York City.  Substrate is a

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On Phytolacca old stem
Elsa Sousa, 06-12-2018 00:02
Good evening,

I need your sharp eyes on this ascos.

Spores mesuring:
(18.3) 20.4 - 27.3 (30.1) × (6.1) 6.5 - 8.2 (9.3) µm
Q = (2.1) 2.7 - 4 (4.4) ; N = 70
Me = 23.7 × 7.4 µm ; Qe = 3.2
2 big guttules, with a 1 septa (?).  some with smaller aditional guttules on tops.

asci 118.2 × 19.2 µm inamyloid

Paraphyses on first observation with refractive tips.

More photos here https://mushroomobserver.org/349254


Would apreciate any ideas.
Thank you very much.
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Hans-Otto Baral, 06-12-2018 08:25
Hans-Otto Baral
Re : On Phytolacca old stem
Wonderful collection in good development! regrettably your equipment is too bad... 

The paraphyses contain VBs whch could point to a Calycina. But with such big spores and asci?!

The spores look non-septate, otherwise the two big drops would be more close in the middle.

Could you please give the apothecial size?

Zotto
Elsa Sousa, 06-12-2018 20:36
Re : On Phytolacca old stem
Hi Zotto,

Thank you for helping with such a terrible means, but that's what my wallet can afford by now.

Apothecia are pink and the larger have about 0.9 mm.
Asci with "crochet".

Elsa
Lothar Krieglsteiner, 06-12-2018 22:16
Lothar Krieglsteiner
Re : On Phytolacca old stem

Hi Elsa,


the apos are pink? So - perhaps an Ombrophila?


Greetings, Lothar

Hans-Otto Baral, 06-12-2018 22:33
Hans-Otto Baral
Re : On Phytolacca old stem
Indeed, we know little about the excipular structure.
Elsa Sousa, 06-12-2018 22:38
Re : On Phytolacca old stem
Hi Lothar,

Let me correct, they are pinkish under scope (light pink for my surprise); scurfy like Calycina pictures, but... at naked eye, they are very tiny and I thought they were yellowish. I thought it wouldn't worth a while scoping those, because I wouldn't see anything with my poor microscope, but I was wrong.

So, I looked for Calycina... the only one macro similar ascomycete that I found, but the spores are too large...so  I decided to upload here.

I'm uploading Iodine reaction. I could only see one tip reacting, so I considered inamyloid, but it could be faintly amyloid.


Thank you.

Elsa
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Hans-Otto Baral, 06-12-2018 23:00
Hans-Otto Baral
Re : On Phytolacca old stem
Difficult to say. You used Lugol? Maybe your Lugol is too diluted? One must see the yellow to reddish stain to the plasma to be sure.

Did you mount directly in your Lugol to get the maximum concentration?
Elsa Sousa, 07-12-2018 00:03
Re : On Phytolacca old stem
Dear Zotto,

First 2 essays I diluted my 10% Iodine solution a bit, some minutes ago I repeated with pure solution and I can't see any change in any of the ascos (only a faintly general blueing), however, there's always a possibility of the solution being old, so... I have to try it with something really amyloid, that I haven't now.

Elsa
Hans-Otto Baral, 07-12-2018 10:07
Hans-Otto Baral
Re : On Phytolacca old stem
10% iodine is impossible, 1% is already a strong solution and requires presence of KI, in water alone you get only maybe 0.2% I2 into solution.

In Melzer's I don't know how much iodine is soluble, but the usual concentration is around 0.5-1% I2.

In both IKI and MLZ the solution is dark red-brown when viewed in a small glass bottle, see under www.in-vivo-veritas.de -- articles -- iodine reaction (last photo).
Elsa Sousa, 11-12-2018 23:24
Re : On Phytolacca old stem

Zotto, Sorry.. I have been busy.

1. I use iodovinilpirrolidona used in desinfection, known by Betadine, or in this case Braunol. The container says 10% available iodine. That's why I'm used to dilute it. I know it's an old product, so I had to test with something amyloid (it always reacted with this solution - i dilute just a bit when I'm mounting the fungus).

2. I found thid Peziza this weekend, I tested with pure solution (diluted too, reaction can be seen) uploaded picture shows reaction. I tested a Otidea and got no reaction. The product must be weak. 

3. Ectal excipulum of this fungus is "intricata".


4. The dead spores end up 1-septate.


Thank you.
Elsa
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Hans-Otto Baral, 12-12-2018 08:11
Hans-Otto Baral
Re : On Phytolacca old stem
Never heard of such an iodine solution, I only now the name Polyvinylpyrrolidin.

Be careful with septa: I suppose this is not a septum but results from the nucleus when the two halfs of the sporoplasm and their ol drops form two confluent bodies, leaving the middle free.